Int J Sport Nutr Exerc Metab. 2014 Jun 5. [Epub ahead of print] No Effect of Short-Term Green Tea Extract Supplementation on Metabolism at Rest or During Exercise in the Fed-State. Martin BJ1, Tan RB, Gillen JB, Percival ME, Gibala MJ. Author information Abstract Supplementation with green tea extract (GTE) in animals has been reported to induce numerous metabolic adaptations including increased fat oxidation during exercise and improved performance. However, data regarding the metabolic and physiological effects of GTE during exercise in humans are limited and equivocal. PURPOSE: To examine the effects of short-term GTE treatment on resting energy expenditure (REE), whole-body substrate utilization during exercise and time trial performance. METHODS: Fifteen active men (24 ± 3 y; VO2peak = 48 ± 7 ml·kg·min-1; BMI = 26 ± 3 kg·m2(-1) ) ingested GTE (3 x/d = 1,000 mg/d) or placebo (PLA) for 2 d in a double-blind, crossover design (each separated by a 1 wk wash-out period). REE was assessed in the fasted state. Subjects then ingested a standardized breakfast (~5.0 kcal·kg-1) and 90 min later performed a 60 min cycling bout at an intensity corresponding to individual maximal fat oxidation (44 ± 11% VO2peak), followed by a 250 kJ TT. RESULTS: REE, whole-body oxygen consumption (VO2) and substrate oxidation rates during steady-state exercise were not different between treatments. However, mean heart rate (HR) was lower in GTE vs. PLA (115 ± 16 vs. 118 ± 17 beats·min-1; main effect, P = 0.049). Mixed venous blood [glycerol] was higher during rest and exercise after GTE vs. PLA (P = 0.006, main effect for treatment) but glucose, insulin and free-fatty acids were not different. Subsequent time trial performance was not different between treatments (GTE = 25:38 ± 5:32 vs. PLA = 26:08 ± 8:13 min:sec; P = 0.75). CONCLUSION: GTE had minimal effects on whole-body substrate metabolism but significantly increased plasma glycerol and lowered heart rate during steady-state exercise, suggesting a potential increase in lipolysis and a cardiovascular effect that warrants further investigation.